Development and validation of a mismatch amplification mutation PCR assay to monitor the dissemination of an emerging variant of Vibrio cholerae O1 biotype El Tor
Open Access
- 28 June 2008
- journal article
- Published by Wiley in Microbiology and Immunology
- Vol. 52 (6) , 314-317
- https://doi.org/10.1111/j.1348-0421.2008.00041.x
Abstract
A mismatch amplification mutation PCR assay was developed and validated for rapid detection of the biotype specific cholera toxin B subunit of V. cholerae O1. This assay will enable easy monitoring of the spread of a new emerging variant of the El Tor biotype of V. cholerae O1.Keywords
This publication has 13 references indexed in Scilit:
- Detection of Cholera Toxin GenesPublished by American Society for Microbiology ,2014
- Genomic analysis of the Mozambique strain of Vibrio cholerae O1 reveals the origin of El Tor strains carrying classical CTX prophageProceedings of the National Academy of Sciences, 2007
- Cholera Due to Altered El Tor Strains of Vibrio cholerae O1 in BangladeshJournal of Clinical Microbiology, 2006
- Differences in Gene Expression between the Classical and El Tor Biotypes of Vibrio cholerae O1Infection and Immunity, 2006
- Genomic Relatedness of the New Matlab Variants of Vibrio cholerae O1 to the Classical and El Tor Biotypes as Determined by Pulsed-Field Gel ElectrophoresisJournal of Clinical Microbiology, 2005
- New Variants of Vibrio cholerae O1 Biotype El Tor with Attributes of the Classical Biotype from Hospitalized Patients with Acute Diarrhea in BangladeshJournal of Clinical Microbiology, 2002
- Mismatch amplification mutation assay (MAMA): application to the c-H-ras gene.Genome Research, 1992
- Epitopes of the Cholera Family of EnterotoxinsClinical Infectious Diseases, 1987
- Identification ofEscherichia coli heat-labile enterotoxin by means of a ganglioside immunosorbent assay (GM1-ELISA) procedureCurrent Microbiology, 1978
- The Subunits of Cholera Toxin: Structure, Stoichiometry, and FunctionThe Journal of Infectious Diseases, 1976