Evidence for a DNA triplex in a recombination‐like motif: I. Recognition of Watson–Crick base pairs by natural bases in a high‐stability triplex

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Abstract
Data are presented on a triplex type with two parallel homologous strands for which triplex formation is almost as strong as duplex formation at least for some sequences and even at pH 7 and 0.2 M NaCl. The evidence mainly rests upon comparing thermodynamic properties of similar systems. A paperclip oligonucleotide d(A12C4T12C4A12) with two linkers C4 obviously can form a triplex with parallel back‐folded adenine strand regions, because the single melting transition of this complex splits in two transitions by introducing mismatches only in the third strand region. Respectively, a hairpin duplex d(A12C4T12) and a single strand d(A12) form a triplex as a 1:1 complex in which the second adenine strand is parallel oriented to the homologous one in the Watson–Crick paired duplex. In this system the melting temperature Tm of the triplex is practically the same as that of the duplex d(A12)·d(T12), at least within a complex concentration range of 0.2–4.0 µM. The melting behaviour of complexes between triplex stabilizing ligand BePI and the system hairpin duplex plus single strand supports the triplex model. Non‐denaturing gel electrophoresis suggests the existence of a triplex for a system in which five of the twelve A·T*A base triads are substituted by C·G*C base triads. The recognition between any substituted Watson–Crick base pair (X·Y) in the hairpin duplex d(A4XA7C4T7YT4) and the correspondingly replaced base (Z) in the third strand d(A4ZA7) is mutually selective. All triplexes with matching base substitutions (Z = X) have nearly the same stability (Tm values from 29 to 33.5°C), whereas triplexes with non‐matching substitutions (Z ≠ X) show a clearly reduced stability (Tm values from 15 to 22°C) at 2µM equimolar oligonucleotide concentration. Most nucleic acid triple helices hitherto known are limited to homopurine–homopyrimidine sequences in the target duplex. A stable triplex formation is demonstrated for inhomogeneous sequences tolerating at least 50% pyrimidine content in the homologous strands. On the basis of the surprisingly similar thermodynamic parameters for duplex and triplex, and of the fact that this triplex type seems to be more stable than many other natural DNA triplexes known, and on the basis of semiempirical and molecule mechanical calculations, we postulate bridging interactions of the third strand with the two other strands in the triplex according to the recombination motif. This triplex, denoted by us ‘recombination‐like form’, tolerates heterogeneous base sequences. Copyright © 2001 John Wiley & Sons, Ltd. Abbreviations used: CD circular dichroism DF degrees of freedom TFO triplex‐forming oligonucleotide.
Funding Information
  • Bundesministerium für Bildung, Wissenschaft, Forschung und Technologie (BEO 21/0311109)
  • Thüringer Ministerium für Wissenschaft, Forschung und Kultur