Spectrofluorimetric Determination of Opium Alkaloids by Mixed Anhydride Reagent

Abstract
The spectrofluorimetric determination of five major opium alkaloids by malonic acid-acetic anhydride reagent is reported. The fluorescence characteristics of various alkaloidal systems and the effect of different parameters like temperature, time of heating and diluent on the fluorescence intensity are described. The interference of various foreign substances has also been checked.