Organization, distribution, and stability of endogenous ecotropic murine leukemia virus DNA sequences in chromosomes of Mus musculus

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Abstract
The endogenous ecotropic murine leukemia virus DNA content and integration sites were characterized for 54 inbred strains and substrains of mice by restriction enzyme digestion, Southern blotting and hybridization with an ecotropic murine leukemia virus DNA-specific probe. More than 75% of these strains, carried endogenous ecotropic proviruses which were located in at least 29 distinct integration sites in chromosomes of M. musculus. Of these proviruses, 14 have been assigned specific locus designations. Most, but not all, of the endogenous ecotropic proviruses were structurally indistinguishable by this analysis from the prototype AKR ecotropic virus, and the distribution of these proviruses followed known relationships among the inbred strains and substrains of mice. In general, viral DNA integration apparently preceded the establishment of inbred mouse strains and that these integrations are relatively stable.