Parallel Synthesis and Screening of a Solid Phase Carbohydrate Library
- 29 November 1996
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 274 (5292) , 1520-1522
- https://doi.org/10.1126/science.274.5292.1520
Abstract
A solid phase carbohydrate library was synthesized and screened against Bauhinia purpurea lectin. The library, which contains approximately 1300 di- and trisaccharides, was synthesized with chemical encoding on TentaGel resin so that each bead contained a single carbohydrate. Two ligands that bind more tightly to the lectin than Gal-β-1,3-GalNAc (the known ligand) have been identified. The strategy outlined can be used to identify carbohydrate-based ligands for any receptor; however, because the derivatized beads mimic the polyvalent presentation of cell surface carbohydrates, the screen may prove especially valuable for discovering new compounds that bind to proteins participating in cell adhesion.Keywords
This publication has 29 references indexed in Scilit:
- Synthesis and Applications of Small Molecule LibrariesChemical Reviews, 1996
- Recognition Specificity of Neoglycopolymers Prepared by Ring-Opening Metathesis PolymerizationJournal of the American Chemical Society, 1996
- Polymer-Supported Solution Synthesis of Oligosaccharides Using a Novel Versatile Linker for the Synthesis of D-Mannopentaose, a Structural Unit of D-Mannans of Pathogenic YeastsJournal of the American Chemical Society, 1995
- Glycosylation on the Merrifield Resin Using Anomeric SulfoxidesJournal of the American Chemical Society, 1994
- Applications of Combinatorial Technologies to Drug Discovery. 2. Combinatorial Organic Synthesis, Library Screening Strategies, and Future DirectionsJournal of Medicinal Chemistry, 1994
- Selection of single-stranded DNA molecules that bind and inhibit human thrombinNature, 1992
- A new type of synthetic peptide library for identifying ligand-binding activityNature, 1991
- Generation and use of synthetic peptide combinatorial libraries for basic research and drug discoveryNature, 1991
- In vitro selection of RNA molecules that bind specific ligandsNature, 1990
- Systematic Evolution of Ligands by Exponential Enrichment: RNA Ligands to Bacteriophage T4 DNA PolymeraseScience, 1990