Immunochemical detection of ADP‐ribosylating enzymes in the archaeon Sulfolobus solfataricus
- 8 January 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 378 (2) , 199-201
- https://doi.org/10.1016/0014-5793(95)01455-1
Abstract
Polyclonal antibodies raised against eukaryotic mono(ADPribose)transferase and poly(ADPribose)polymerase were used to test the presence of antigenic determinants in a crude extract of Sulfolobus solfataricus, a thermophilic archaeon. Samples from eukaryotic (bull testis) and bacterial (E. coli) sources were analysed for comparison. All tested antibodies reacted with the sulfolobal sample with a specificity comparable to that of the eukaryotic preparation, as revealed by ELISA test, activity assays in the presence of antibodies and immunoblot experiments. After electrophoresis and western blot of sulfolobal proteins, a band at a mass around 50 kDa was detected by immunostaining.Keywords
This publication has 10 references indexed in Scilit:
- In the Thermophilic Archaeon Sulfolobus solfataricus a DNA-Binding Protein Is in Vitro (Adpribosyl)AtedBiochemical and Biophysical Research Communications, 1995
- ADP-ribosylation reactions in Sulfolobus solfataricus, a thermoacidophilic archaeonBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1995
- Evolution: Archaea and eukaryotes versus bacteria?Current Biology, 1994
- Immunological and structural conservation of mammalian skeletal muscle glycosylphosphatidylinositol-linked ADP-ribosyltransferasesBiochemistry, 1994
- Loss of poly(ADP-ribose) synthetase activity without change in arginine-specific ADP-ribosyltransferase activity by acid-treatment; application of the treatment to simple assay for transferase in the presence of poly(ADP-ribose) synthetaseBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1993
- Molecular and biochemical features of poly (ADP-ribose) metabolismMolecular and Cellular Biochemistry, 1993
- ADP-Ribosylation ReactionsPublished by Springer Nature ,1992
- Detection of poly(ADP ribose) polymerase in crude extracts by activity-blotAnalytical Biochemistry, 1991
- Computer modelling of the NAD binding site of ADP‐ribosylating toxins: active‐site structure and mechanism of NAD bindingMolecular Microbiology, 1991
- Structural and functional analysis of poly(ADP ribose) polymerase: an immunological studyBiochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1988