Isolation and partial characterization of a lipopolysaccharide from phase II Coxiella burnetii

Publisher Website
Google Scholar
Abstract
A lipopolysaccharide (LPS) was isolated by hot phenol-water extraction from phase II C. burnetii. The LPS was isolated from organism that had a history of 95 serial yolk sac passages and others that were cloned. The chemical composition of the LPS was partially determined and compared with that of LPS previously isolated from phase I organisms. Most of the sugars present in phase I LPS were detected in phase II LPS, but there were some quantitative and qualitative differences. The fatty acid profiles of phase I and II LPS were identical. The amount of LPS extracted from phase II organisms was .apprx. 1/10 the amount extracted from phase I C. burnetii. Limulus lysate gelation was observed with subnanogram amounts of both phase I and II LPS. In complement fixation tests, both LPS reacted only with antisera containing antibodies vs. phase I antigen. Ouchterlony immunodiffusion tests demonstrated immunological identity of the 2 LPS.