Effect of Substituted Benzimidazoles on H+K+ATPase of Isolated Guinea-pig Parietal Cells

Abstract
Omeprazole, a substituted benzimidazole, has been shown to inhibit gastric acid secretion in vitro and in vivo. In vitro, omeprazole inhibits acid secretion stimulated at all cellular levels, including that stimulated by K+, indicating that it is an inhibitor of the parietal cell proton pump. The present paper describes the inhibitory effect of substituted benzimidazoles, such as picoprazole and omeprazole, on the H+K+ATPase isolated from guinea-pig parietal cells, and the reversibility of acid inhibition in isolated guinea-pig parietal cells by the thiol compound, dithiothreitol.