Ribonucleotide reductase genes of Bacillus prophages: a refuge to introns and intein coding sequences
Open Access
- 1 August 2001
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 29 (15) , 3212-3218
- https://doi.org/10.1093/nar/29.15.3212
Abstract
The ribonucleotide reductase gene tandem bnrdE/bnrdF in SPβ-related prophages of different Bacillus spp. isolates presents different configurations of intervening sequences, comprising one to three of six non-homologous splicing elements. Insertion sites of group I introns and intein DNA are clustered in three relatively short segments encoding functionally important domains of the ribonucleotide reductase. Comparison of the bnrdE homologs reveals mutual exclusion of a group I intron and an intein coding sequence flanking the codon that specifies a conserved cysteine. In vivo splicing was demonstrated for all introns. However, for two of them a part of the mRNA precursor molecules remains unspliced. Intergenic bnrdE–bnrdF regions are unexpectedly long, comprising between 238 and 541 nt. The longest encodes a putative polypeptide related to HNH homing endonucleases.Keywords
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