Bacteriophage CP‐T1 of Vibrio cholerae
- 1 November 1985
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 153 (1) , 89-94
- https://doi.org/10.1111/j.1432-1033.1985.tb09271.x
Abstract
The attachment site on the cell surface of Vibrio cholerae for the bacteriophage CP-T1 has been determined. Purified lipopolysaccharide from the Inaba and Ogawa serotypes, and of both the Classical and El Tor biotype of strains of V. cholerae show equal phage-inactivating capacities. Lipopolysaccharide extracted from a CP-T1-resistant mutant has no phage-inactivating capacity. Such mutants lack O-antigen as demonstrated by bactericidal assays utilizing a monoclonal antibody directed against O-antigen side chain of V. cholerae lipopolysaccharide. Radiolabelling of lipopolysaccharide with 33P and analysis by sodium dodecyl sulfate/polyacrylamide gel electrophoresis also revealed the absence of O-antigen in phage-resistant strains. A number of V. cholerae typing phage show cross-resistance with phage CP-T1.This publication has 26 references indexed in Scilit:
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