Stoichiometry of metal‐tetracycline/H+ antiport mediated by transposon Tn10‐encoded tetracycline resistance protein in Escherichia coli
- 6 May 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 282 (2) , 415-418
- https://doi.org/10.1016/0014-5793(91)80527-a
Abstract
The tetracycline resistance protein (TetA) endoded by transposon Tn10 mediates the efflux of divalent cation-tetracycline chelating complexes [Yamaguchi, A., Udagawa, T. and Sawai, T. (1990) J. Biol. Chem. 265, 4809-4813]. It was confirmed that protons were antiported with the complexes through an electrically-neutral process because the antiport consumed delta pH but not delta psi. The quantitative relationship between delta pH and delta pTC determined by a flow-dialysis method clearly indicated a 1:1 stoichiometry of the monocationic metal-tetracycline/H+ exchange.Keywords
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