Elevated HGF Levels in Sera from Breast Cancer Patients Detected Using a Protein Microarray ELISA
- 18 April 2002
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Proteome Research
- Vol. 1 (3) , 233-237
- https://doi.org/10.1021/pr025506q
Abstract
We developed an ELISA in high-density microarray format to detect hepatocyte growth factor (HGF) in human serum. The microassay can detect HGF at sub-pg/mL concentrations in sample volumes of 100 μL or less. The microassay is also quantitative and was used to detect elevated HGF levels in sera from recurrent breast cancer patients. The microarray format provides the potential for high-throughput quantitation of multiple biomarkers in parallel, as demonstrated with a multiplex analysis of five biomarker proteins. Keywords: microarray • ELISA • breast cancer • hepatocyte growth factorKeywords
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