The Assimilation of N15-from Labeled Hyponitrite by Soybean Leaves.

Abstract
Labeled sodium hypo-nitrite was prepared in low yield with high purity by the reduction of Nl5 labeled sodium nitrate with sodium amalgam. Aqueous solutions of sodium hyponitrite were found to be quite stable above pH 7.0. Labeled hyponitrite nitrogen was assimilated by infiltrated soybean leaf sections in the light and in the dark. Hyponitrite nitrogen was partially or completely oxidized to the level of nitrite or nitrate prior to its reduction to the level of ammonia by infiltrated soybean leaves. The implications of these findings are discussed.