Regulation of Glutamine Synthetase from Saccharomyces cerevisiae by Repression, Inactivation and Proteolysis
- 1 April 1982
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 123 (3) , 611-616
- https://doi.org/10.1111/j.1432-1033.1982.tb06576.x
Abstract
Glutamine synthetase activity is modulated by N repression and by 2 distinct inactivation processes. Addition of glutamine to exponentially grown yeast leads to enzyme inactivation. 50% of glutamine synthetase activity is lost after 30 min (a quarter of the generation time). Removing glutamine from the growth medium results in a rapid recovery of enzyme activity. A regulatory mutation (gdhCR mutation) suppresses this inactivation by glutamine in addition to its depressing effect on enzymes involved in N catabolism. The gdhCR mutation also increases the level of proteinase B in exponentially grown yeast. Inactivation of glutamine synthetase is also observed during N starvation. This inactivation is irreversible and probably consists of a proteolytic degradation. Strains bearing proteinase A, B and C mutations are no longer inactivated under N starvation.This publication has 40 references indexed in Scilit:
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