RNA Synthesis in Starved Deciliated Tetrahymena pyriformis

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Abstract
T. pyriformis starved for 20 h by incubation in buffer, and then deciliated, can regenerate its cilia in about 90 min while still in suspension in non-nutrient medium. The process of reciliation is accompanied by protein synthesis which begins a few minutes after deciliation and by synthesis of rRNA and mRNA during a period extending from about 1 h to about 3 h after deciliation. Although net synthesis of RNA remains at a very low level until 1 h after deciliation, a qualitative change in the translatable poly(A)-containing mRNA content of deciliated cells, and in particular, formation of .beta.-tubulin mRNA can be detected almost immediately after deciliation.