Studies on the Development of Dirofilaria tenuis Chandler 1942

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Abstract
Sixteen species of mosquitoes were screened to determine their ability to maintain to infectivity the larval stages of Dirofilaria tenuis. Development occurred readily in the malphigian tubules of Aedes taeniorhynchus and Anpheles quadrimaculatus. Molts occurred on the 7-8 th and 9-10 th days after feeding on an infected raccoon. Migration to the proboscis via the haemocoel after the tubules and ventriculus had been actively penetrated was observed. Two of 4 experimentally exposed raccoons developed the infection. Adults were recovered 7 and 14 months after the experimental exposure. Attempts to infect 4 dogs were unsuccessful. Raccoon subcutaneous tissues recovered from the site of infection with adult worms showed a thin capsule around the nematode, and the reaction observed was very slight and generally considered negligible.
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