Immunodiagnosis of Bovine Fascioliasis by Enzyme-Linked Immunosorbent Assay and Immunoprecipitation Methods

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Abstract
Calves were infected with 1000 Fasciola hepatica metacercariae and the antibodies which developed were monitored by gel diffusion, counterelectrophoresis (CEP) and the enzyme-linked immunosorbent assay (ELISA) using crude or tegument antigen preparations of the adult fluke. Serologic diagnosis was positive as early as 2-4 wk of infection by gel diffusion, by 4 wk by CEP, and by 6-8 wk by ELISA, whereas parasitologic diagnosis via the detection of fluke eggs in the feces was possible only after 8-10 wk of infection. Both gel diffusion and ELISA were superior to CEP in detecting active infection.