Metabolism of N-Nitrosohexamethyleneimine To Give 1,6-Hexanediol Bound to Rat Liver Nucleic Acids23
- 1 March 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in JNCI Journal of the National Cancer Institute
- Vol. 58 (3) , 651-655
- https://doi.org/10.1093/jnci/58.3.651
Abstract
Rats were gavaged with the liver carcinogen N-nitrosohexamethyleneimine labeled with 3H or 14C and killed 16 hours later. The liver RNA and DNA were isolated and hydrolyzed with 1 M HCI at 100°C. Chromatography of the 3H-labeled RNA hydrolysate on a cation exchange resin (NH4+ form), with water elution, separated five radioactive peaks, with peak “E” containing 27% of the bound 3H. There were no radioactive peaks in the 7-substituted guanine region. Hydrolysis of the 3H-labeled DNA gave a similar profile, but E contained only 5% of the 3H. The major component of E was identified as 1,6-hexanediol by its behavior and/or that of its benzoate derivative on cation exchange, anion exchange, thin-layer and gas-liquid chromatography, and by recrystallization of a mixture of the E and diol benzoates to constant specific radioactivity.Keywords
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