Use of hybridization kinetics for differentiating specific from non-specific binding to oligonucleotide microarrays
Open Access
- 15 August 2002
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 30 (16) , 86e-86
- https://doi.org/10.1093/nar/gnf085
Abstract
Hybridization kinetics were found to be significantly different for specific and non‐specific binding of labeled cRNA to surface‐bound oligonucleotides on microarrays. We show direct evidence that in a complex sample specific binding takes longer to reach hybridization equilibrium than the non‐ specific binding. We find that this property can be used to estimate and to correct for the hybridization contributed by non‐specific binding. Useful applications are illustrated including the selection of superior oligonucleotides, and the reduction of false positives in exon identification.Keywords
This publication has 23 references indexed in Scilit:
- Functional Discovery via a Compendium of Expression ProfilesCell, 2000
- Distinct types of diffuse large B-cell lymphoma identified by gene expression profilingNature, 2000
- High density synthetic oligonucleotide arraysNature Genetics, 1999
- Expression monitoring by hybridization to high-density oligonucleotide arraysNature Biotechnology, 1996
- Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA MicroarrayScience, 1995
- Complete Genomic Sequence of the Human Retinoblastoma Susceptibility GeneGenomics, 1993
- A human DNA segment with properties of the gene that predisposes to retinoblastoma and osteosarcomaNature, 1986
- Slow relaxational processes in the melting of linear biopolymers: A theory and its application to nucleic acidsBiopolymers, 1984
- Comparison of the kinetics of ribo-, deoxyribo- and hybrid oligonucleotide double-strand formation by temperature-jump kineticsBiochemistry, 1982
- Kinetics of renaturation of DNAJournal of Molecular Biology, 1968