Stimulation of in Vitro Translation of Messenger RNA by Actinomycin D and Cordycepin

Abstract
Actinomycin D and cordycepin were tested for their effect on translation in the wheat germ embryo extract and reticulocyte lysate assays for in vitro protein synthesis. Both drugs were found to stimulate the incorporation of 35S-labeled methionine into protein up to threefold as compared to control assays. This was true for synthesis directed by murine myeloma polyadenylate-containing RNA, tobacco mosaic virus RNA, and endogenous reticulocyte messenger RNA.