Enrichment of Human X- and Y-Chromosome Bearing Spermatozoa by Density Gradient Centrifugation
- 24 April 2009
- journal article
- Published by Wiley in Andrologia
- Vol. 9 (1) , 106-110
- https://doi.org/10.1111/j.1439-0272.1977.tb01269.x
Abstract
Human spermatozoa (120--180 Mill/0.6 ml Tyrode solution) were laid on the top of a continous density gradient column consisting of 4--16% bovine serum albumin. The columns were then centifuged at 1500--1600 rpm (200-240 g) at 20 degrees C or 4 degrees C for 15 min. By this procedure the spermatozoa sedimented within the tubes of normal distribution curves. The mean percentages of y-spermatozoa gradually decreased from 62,3 +/- 6,1% (max. 70%) in the upper fractions to 42,4 +/- 3,8% (min. 37%) in the bottom fractions. These results could be verified by inseminations of rabbit does with spermatozoa separated by the same technique.Keywords
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