In Vitro Evaluation of Circadian Patterns of Bone Collagen Formation

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Abstract
The circadian patterns of bone collagen formation were studied in vitro to ascertain whether the biorhythmic profiles previously measured in vivo reflect the true differences in collagen synthesis. Alteration of amino acid pool sizes did not negate the circadian-stage differences in bone collagen production. Evaluations of proline uptake and transport, as well as collagenous protein turnover, demonstrated that the intracellular assembly and secretion of bone collagenous protein during the dark span is truely decreased relative to that during the light period. It was further affirmed that PTH is essential for maintainance of the normal circadian collagen synthesis rhythms.