Distribution of Some Phosphohydrolases in Glomerular and Tubular Fractions Isolated from Rabbit Kidney

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Abstract
The general chemical composition of the glomerular and tubular fractions isolated from the renal cortex of the rabbit and the distribution of some phosphohydrolases are described. The protein and lipid content, expressed in mg for 100 mg dry weight, is roughly the same in both fractions. On the other hand, the RNA,/DNA, ratio is about three times less in the glomeruli than in the tubules. 5′-mononucleotidase is totally absent in the glomerular fraction. Specific activity of Na− + K+-activated, ouabain-sensitive and of alkaline phosphatase in the glomeruli represents only about 4% of that of the tubules. The glomerular activity of Mg2+-activated ATPase represents about 44% of that of the tubules. Acid phosphatase is 3.5 times more active in the glomerular fraction than in the tubules. Alkaline phosphatase, 5′-mononucleotidase and Na+ + K+-activated, ouabain-sensitive ATPase may be used as biochemical markers to assess the purity of glomerular fractions.