Chloride, sodium, potassium and hydrogen ion transport in isolated canine gastric mucosa.

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Abstract
The fluxes of isotopically labeled Na+, Cl- and K+ in each direction and H+ secretion across isolated dog gastric mucosa were measured under short-circuit conditions. In the non-stimulated state, the net flux of Na+ was 6.61 .mu.equiv/cm2 .cntdot. h from mucosal (luminal, secretory) to serosal (nutrient, blood) side, whereas the net flux of Cl- was only 0.79 .mu.equiv/cm2 .cntdot. h and the direction was from serosal to mucosal side. There was a positive correlation between the net flux of Cl- and acid secretion. Net flux of Na+ was not correlated with acid secretion initiated by secretagogue treatment. With ion substitution studies, only replacement of mucosal Na+ with choline produced a highly significant decrease in potential difference (p.d.). Active transport of Na+ from the mucosal to the serosal side is the most important source for the generation of the gastric p.d. in dog gastric mucosa. While Cl- in either mucosal or serosal solution is necessary for maintaining acid secretion, only serosal Na+ and K+ are essential for acid secretion. Removal of either Na+ or K+ from the mucosal solution had no effect on acid secretion. Substitution of SO42- for Cl- had no effect on active transport of Na+, but choline substitution for Na+ diminished active transport of Cl-.

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