OXIDIZING ENZYMES IN BRAIN EXTRACTS

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Abstract
A colloidal sol of rabbit brain, prepared by cytolysis in 10 volumes of water, respires at about 1/10 the rate of uncytolyzed brain. Added methyl glyoxal, glycerophosphate, lactate, fructose and, especially, succinate and p-phenylenediamine are oxidized. Succinate is oxidized several times more rapidly than by intact brain. Lactate oxidation is diminished, due to partial separation of components of the catalytic system in sol and residue. Some heat stable oxidation of lactate occurs. There is no evidence that succinate-fumarate serves as a carrier for lactate oxidation in the sol. Glucose oxidation disappears as structure is destroyed. Cyanide inhibits these oxidations (but accelerates that of methyl glyoxal) and methylene blue partially restores some of them. The succinic dehydrogenase and cytochrome-oxidase systems are precipitated from the sol at pH 4.6 without inactivation. This permits a 6- to 8-fold concn. of the enzymes.

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