The Effect of pH at Different Temperatures on the Growth of Bacterium coli with a Constant Food Supply

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Abstract
The effect of pH over the range of 5-9 on the growth of Escherichia coli with a constant food supply was studied at 20 to 30 C. The apparatus was previously described. Total counts always substantially exceeded the viable. The initial phase of growth, in which the daily increments in the numbers of total and viable cells changed rapidly, was shortened by low pH and prolonged by low temp. During the steady phase, in which the daily increments were approx. constant, low temp. and low pH gave max. yield of cell substance. During starvation, after additions to the food supply had been stopped, the total counts declined except at 30 C and pH 5.

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