Formylmethanofuran dehydrogenase from methanogenic bacteria, a molybdoenzyme
- 14 August 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 253 (1-2) , 226-230
- https://doi.org/10.1016/0014-5793(89)80964-0
Abstract
Formylmethanofuran dehydrogenase, a key enzyme of methanogenesis, was purified 100‐fold from methanol grown Methanosarcina barkeri to apparent homogeneity and a specific activity of 34 μmol·min−1·mg protein−1. Molybdenum was found to co‐migrate with the enzyme activity. The molybdenum content of purified preparations was 3–4 nmol per mg protein equal to 0.6–0.8 mol molybdenum per mol enzyme of apparent molecular mass 200 kDa. Evidence is presented that also formylmethanofuran dehydrogenase from H2/CO2 grown Methanobacterium thermoautotrophicum (strain Marburg) is a molybdoenzyme.Keywords
This publication has 20 references indexed in Scilit:
- Methyltetrahydromethanopterin as an intermediate in methanogenesis from acetate in Methanosarcina barkeriArchiv für Mikrobiologie, 1989
- Formylmethanofuran dehydrogenase activity in cell extracts of Methanobacterium thermoautotrophicum and of Methanosarcina barkeriFEBS Letters, 1989
- Methanogenesis and ATP synthesis in methanogenic bacteria at low electrochemical proton potentialsEuropean Journal of Biochemistry, 1988
- The final step in methane formationEuropean Journal of Biochemistry, 1988
- [29] MolybdenumPublished by Elsevier ,1988
- Mechanistic studies of the coenzyme F420-reducing formate dehydrogenase from Methanobacterium formicicumBiochemistry, 1986
- Biochemistry and physiology of aerobic carbon monoxide-utilizing bacteriaFEMS Microbiology Letters, 1986
- Purification and Properties of Reduced Ferredoxin: CO2 Oxidoreductase from Clostridium pasteurianum, a Molybdenum Iron‐Sulfur‐ProteinEuropean Journal of Biochemistry, 1978
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976