In vitro protein translocation into inverted membrane vesicles prepared from Vibrio alginolyticus is stimulated by the electrochemical potential of Na+ in the presence of Escherichia coli SecA
- 7 May 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 264 (1) , 10-12
- https://doi.org/10.1016/0014-5793(90)80751-4
Abstract
A protein translocation system was reconstituted from inverted membrane vesicles prepared from Na+ pump-possessing Vibrio alginolyticus and purified Escherichia coli SecA. The translocation required ATP and was stimulated by the functioning of the Na+ pump, suggesting that the electrochemical potential of Na+ but not that of H+, is important for protein translocation in Vibrio.Keywords
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