Fluorescent in situ Hybridization for the Detection of t(8:14) in Burkitt’s Lymphoma
- 1 January 1993
- journal article
- case report
- Published by S. Karger AG in Acta Haematologica
- Vol. 90 (4) , 186-189
- https://doi.org/10.1159/000204455
Abstract
Burkitt’s lymphoma cells exhibit the reciprocal balanced chromosomal translocation t(8:14) in 75% of patients. Cytogenetic analysis is time-consuming, requires in vitro culture with mitogens and enables to analyse a relatively small number of cells. We evaluated the role of multicolor fluorescent in situ hybridization (FISH) in the rapid detection of t(8:14) in Burkitt’s lymphoma cells. FISH detected the JH/myc translocation in 100% of the cells of five cell lines carrying the classical t(8:14) and in fresh cells obtained from a newly diagnosed Burkitt’s lymphoma patient. In contrast, the translocation was not detected in the Bajb cell line that does not carry t(8:14). We conclude that FISH is a rapid and reliable diagnostic tool for the detection of the JH/myc translocation in Burkitt’s lymphoma patients.Keywords
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