CONTINUOUS-FLOW ENZYMATIC DETERMINATION OF TOTAL SERUM-CHOLESTEROL AND METHOD STANDARDIZATION WITH CDC-CALIBRATED POOLED SERA

  • 1 January 1980
    • journal article
    • research article
    • Vol. 26  (7) , 896-902
Abstract
Boehringer Mannheim''s enzymatic kit for the continous-flow (AutoAnalyzer II) determination of serum cholesterol was compared with Technicon''s N-24a extraction method. Results for patients'' samples analyzed by the enzymatic method were higher than those by the comparison method. To evaluate accuracy in the cholesterol determinations, the enzymatic method was enrolled into the Center for Disease Control''s (CDC) Lipid Standardization program. The method was calibrated by use of a pooled sera for which cholesterol content was assigned by CDC after analysis by their reference Abell-Kendall procedure. The difficulties with available calibration material and limitations in the application of some commercial control materials to the enzymatic cholesterol method were discussed. The continuous-flow variables, Michaelis-Menton constants, percent ester-hydrolase activity and other factors affecting the performance of the enzyme-linked cholesterol method are evaluated. Pooled sera with an assigned value for cholesterol content is apparently the best calibrator material.