Calvasculin, as a factor affecting the microfilament assemblies in rat fibroblasts transfected by src gene
- 7 June 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 324 (1) , 51-55
- https://doi.org/10.1016/0014-5793(93)81530-d
Abstract
Cell transformations accompany alterations in cell morphology and microfilament patterns. Calvasculin encodes mRNA termed pEL‐98, 18A2, 42A, p9Ka, or mtsl, found to be elevated in several metastatic cell lines. We report the elevation of calvasculin expression in SR‐3Y1 cells, which show disappearance of ordered microfilaments, compared to that in 3Y1 cells and that the similar distribution of calvasculin to that of actin filaments. Interestingly, calvasculin co‐sediments with F‐actin and bundles actin filaments in a Ca2+‐dependent manner. This activity, along with the elevation of calvasculin following transformation, suggests that the disorganization of filaments in SR‐3Y1 cell is due to the cross‐linking activity of calvasculin.Keywords
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