In vitro study on release of thyroid hormone in solitary autonomously functioning thyroid nodules using cell culture method.

Publisher Website
Google Scholar
Abstract
In vitro release of thyroid hormone was investigated under basal and TSH-stimulated conditions in the solitary autonomously functioning thyroid nodules (AFTN). A small portion (0.5 g of wet wt) of the nodules and adjacent thyroid tissues removed surgically from 5 patients with solitary AFTN were prepared for the dispersed cell culture. In the experiment on non TSH-stimulated (basal) conditions, those culture media which were totally replaced on the 5th day after primary culture were utilized for the determination of thyroxine (T4) and triiodothyronine (T3) by radioimmunoassay. T4 and T3 levels in culture media of the functioning nodules were 1.15 .+-. 0.33 .mu.g/dl (mean .+-. SEM [standard error of the mean]) and 2.72 .+-. 0.68 ng/ml, contrasted with levels of 0.67 .+-. 0.09 .mu.g/dl and 1.24 .+-. 0.22 ng/ml in the paranodular tissues. The mean ratios of T3/T4 of the nodules and paranodular tissues were 0.25 .+-. 0.02 and 0.19 .+-. 0.02, respectively, (P < 0.05). Meanwhile, in another experiment under TSH stimulatory conditions employing 40 and 80 .mu.U/ml of human TSH, there were no significant differences in T4 and T3 releases when the 2 groups were compared.

This publication has 1 reference indexed in Scilit: