Production ofBartonellaGenus-Specific Monoclonal Antibodies

Abstract
Monoclonal antibodies (MAbs) which react with heat-resistant proteins with molecular masses of 32 to 33 kDa of 14 differentBartonellaspecies were produced. These antibodies did not react with antigens of 26 diverse bacterial strains by microimmunofluorescence assay except MAb B3D4, which reacted withChlamydia psittaciandChlamydia trachomatisat low titers. The identification of a commonBartonellaantigenic protein will make it possible to later produce a diagnostic antigen by cloning and expressing it inEscherichia coli. Moreover, these MAbs allow allBartonellaspecies to be identified to the genus level.

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