ZONATION OF ADRENAL-CORTEX .3. DISTRIBUTION OF CYTOCHROME-P-450 IN ZONA GLOMERULOSA OF BOVINE ADRENAL-CORTEX
- 1 January 1976
- journal article
- research article
- Vol. 26 (4) , 467-479
Abstract
A microsomal fraction was obtained from the zona glomerulosa of the bovine adrenal cortex. Glucose-6-phosphatase activity of the fraction was much lower than that of the liver. Contents of RNA and phospholipids, besides EM findings, of the fraction also indicate that it is rich in smooth-surfaced endoplasmic reticulum. Distribution of cytochrome P-450 in the zona glomerulosa was studied using various fractions including the microsomal fraction. The amount of cytochrome P-450 in mitochondria and that in microsomes were 0.73 and 0.32 nmoles/mg protein, respectively. The CO-difference spectrum was affected not only by the concentration of added deoxycholate but also by the incubation time after addition. Approximately 40-50% of cytochrome P-450 in the samples were converted to cytochrome P-420 within 20-30 s of incubation with deoxycholate.This publication has 14 references indexed in Scilit:
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