Comparison of Four Methods for Typing Low-Passage Herpes Simplex Virus Isolates

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Abstract
Clinical isolates of herpes simplex virus (HSV) were identified as HSVtype 1 or type 2 by sensitivity to (E)-5-(2-bromovinyl)-2’-deoxyuridine (BVDU), by differential replication in chick embryo cells versus guinea pig embryo cells, by restriction endonuclease analysis, and by a direct fluorescent antibody technique using monoclonal antibodies. More than 550 isolates were typed by two or three of the systems with complete agreement as to virus type between systems for each isolate. In appropriately equipped laboratories, any of the above typing systems can be used with complete confidence. However, the BVDU sensitivity assay, particularly when used in a continuous cell line as described, can be economically utilized in any virology laboratory.

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