Quantitation of Plasma Aldosterone by Radioimmunoassay
- 1 November 1972
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 18 (11) , 1395-1402
- https://doi.org/10.1093/clinchem/18.11.1395
Abstract
Aldosterone antibodies were produced by injecting sheep with the albumin conjugate of aldosterone-18,21-dihemisuccinate. Conditions optimal for an accurate, reproducible assay with this antiserum were determined experimentally. Aldosterone is extracted from plasma and isolated by a single thin-layer chromatographic system before the immunoassay is performed. Normal plasma aldosterone in supine subjects is 8.6 ± 3.8 ng/100 ml; after 2 h in the upright position, 18.3 ± 9.5 ng/100 ml. Eighteen months of experience with the method have proved it to be reliable with respect to reproducibility, accuracy, and precision.Keywords
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