Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector.
Open Access
- 1 September 1989
- journal article
- research article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 3 (9) , 1273-1287
- https://doi.org/10.1101/gad.3.9.1273
Abstract
A P-element vector has been constructed and used to generate lines of flies with single autosomal P-element insertions. The lines were analyzed in two ways: (1) the identification of cis-acting patterning information within the Drosophila genome, as revealed by a lacZ reporter gene within the P element, and (2) the isolation of lethal mutations. We examined 3768 independent lines for the expression of lacZ in embryos and looked among these lines for lethal mutations affecting embryonic neurogenesis. This type of screen appears to be an effective way to find new loci that may play a role in the development of the Drosophila nervous system.This publication has 56 references indexed in Scilit:
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