Co-overexpression of Escherichia coliRNA Polymerase Subunits Allows Isolation and Analysis of Mutant Enzymes Lacking Lineage-specific Sequence Insertions
Open Access
- 1 April 2003
- journal article
- Published by Elsevier in Journal of Biological Chemistry
- Vol. 278 (14) , 12344-12355
- https://doi.org/10.1074/jbc.m211214200
Abstract
No abstract availableKeywords
This publication has 62 references indexed in Scilit:
- Allosteric Control of RNA Polymerase by a Site That Contacts Nascent RNA HairpinsScience, 2001
- A mutation in the C-terminal domain of the RNA polymerase alpha subunit that destabilizes the open complexes formed at the phage φ29 late A3 promoterJournal of Molecular Biology, 2001
- RNA Polymerase: Structural Similarities Between Bacterial RNA Polymerase and Eukaryotic RNA Polymerase IIJournal of Molecular Biology, 2000
- RNA polymerase structure–function: insights into points of transcriptional regulationCurrent Opinion in Microbiology, 2000
- Multiple interactions stabilize a single paused transcription intermediate in which hairpin to 3′ end spacing distinguishes pause and termination pathwaysJournal of Molecular Biology, 1997
- Mechanism of initiation of transcription by Bacillus subtilis RNA polymerase at several promotersJournal of Molecular Biology, 1992
- Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressorJournal of Molecular Biology, 1991
- Kinetics of open complex formation between Escherichia coli RNA polymerase and the lac UV5 promoter. Evidence for a sequential mechanism involving three stepsBiochemistry, 1985
- The Reconstitution of Escherichia coli DNA-Dependent RNA Polymerase from Its Isolated SubunitsEuropean Journal of Biochemistry, 1975
- Subunits of RNA polymerase in function and structure: V. Maturation in vitro of core enzyme from Escherichia coliJournal of Molecular Biology, 1974