Size-Selective Capillary Electrophoresis (SSCE) Separation of DNA Fragments

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Abstract
A capillary electrophoretic separation of DNA fragment standards in the range of 50 to 105 base pairs was achieved using methylcellulose as a size-selective agent. All 23 fragments in a 1 kb DNA ladder can be resolved in under 25 minutes. Capillary wall deactivation, using a siloxane-bonded polyacrylamide, greatly improved resolution and reproducibility. The concentration effect of the size-selective agent and the applied electric field strength on DNA fragment separation was determined. Instability of the wall coating limits capillary lifetimes to approximately 30 runs. Considerations in using SSCE for the analysis of DNA fragments produced by the PCR technique are discussed.