Expression of hepatitis C virus core protein as a fusion protein with maltose binding protein
- 1 April 1993
- journal article
- Published by Springer Nature in Digestive Diseases and Sciences
- Vol. 38 (4) , 626-630
- https://doi.org/10.1007/bf01316791
Abstract
Putative hepatitis C virus core sequence was amplified from a serum sample positive for anti-C-100-3 and expressed inEscherichia coli. Approximately 62 kDa fusion protein with maltose binding protein containing 20 kDa hepatitis C core protein was obtained. The antibody to this protein was detected in 53 of 54 (98%) sera from hepatitis C virus ribonucleic acid-positive patients including 40 sera positive for anti-C-100-3 and 13 sera negative for anti-C-100-3. The antibody was also detected in all of 12 patients with acute hepatitis C showing the earlier detectability of the antibody than anti-C-100-3. Thus, the protein expressed from the amplified hepatitis C core sequence by the polymerase chain reaction would be useful for the diagnosis of hepatitis C.Keywords
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