Microtubule-Associated-Protein MAP1 is not Implicated in the Polymerization of Microtubules
- 3 March 2005
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 112 (3) , 611-616
- https://doi.org/10.1111/j.1432-1033.1980.tb06126.x
Abstract
Addition of DNA to [porcine brain] microtubule protein solutions results in a decrease in the amount of the high MW microtubule-associated proteins (MAP1 and MAP2) available for polymerization. Complete removal of MAP1 from microtubules requires lower concentrations of DNA compared to MAP2. Removal of MAP1 from microtubules has little or no effect on microtubule polymerization (nucleation and propagation). Removal of MAP2 produces a marked decrease in microtubule polymerization. Incubation of microtubular protein with DNA results in complete disappearance of the 30-S ring-shaped oligomers at concentrations of DNA which are not markedly inhibitory of polymerization.This publication has 32 references indexed in Scilit:
- Identification of the major 68,000-dalton protein of microtubule preparations as a 10-nm filament protein and its effects on microtubule assembly in vitroBiochemistry, 1979
- Purification of a thermostable high molecular weight factor promoting tubulin polymerizationFEBS Letters, 1978
- Role of tubulin-associated proteins in microtubule nucleation and elongationJournal of Molecular Biology, 1977
- Physical and chemical properties of purified tau factor and the role of tau in microtubule assemblyJournal of Molecular Biology, 1977
- Purification of tau, a microtubule-associated protein that induces assembly of microtubules from purified tubulinJournal of Molecular Biology, 1977
- A kinetic analysis of the assembly of microtubules in vitroFEBS Letters, 1977
- Turbidimetric studies of the in vitro assembly and disassembly of porcine neurotubulesJournal of Molecular Biology, 1974
- Electrophoretic analysis of the major polypeptides of the human erythrocyte membraneBiochemistry, 1971
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Size distribution of protein polymersJournal of Theoretical Biology, 1970