Novel Dephosphotetrahydromethanopterin Biosynthesis Genes Discovered via Mutagenesis in Methylobacterium extorquens AM1
Open Access
- 1 April 2005
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 187 (7) , 2508-2512
- https://doi.org/10.1128/jb.187.7.2508-2512.2005
Abstract
Methylobacterium extorquens AM1 was used to explore the genetics of dephosphotetrahydromethanopterin (dH 4 MPT) biosynthesis. Strains with mutations in eight “archaeal-type” genes linked on the chromosome of M. extorquens AM1 were analyzed for the ability to synthesize dH 4 MPT, and six were found to be dH 4 MPT negative. Putative functions of these genes in dH 4 MPT biosynthesis are discussed.Keywords
This publication has 24 references indexed in Scilit:
- Broad-Host-Range cre-lox System for Antibiotic Marker Recycling in Gram-Negative BacteriaBioTechniques, 2002
- Purification, Overproduction, and Partial Characterization of β-RFAP Synthase, a Key Enzyme in the Methanopterin Biosynthesis Pathway†Journal of Bacteriology, 2002
- Generation of formate by the formyltransferase/hydrolase complex (Fhc) from Methylobacterium extorquens AM1FEBS Letters, 2002
- Elucidation of methanogenic coenzyme biosyntheses: from spectroscopy to genomicsNatural Product Reports, 2001
- Development of improved versatile broad-host-range vectors for use in methylotrophs and other Gram-negative bacteria The GenBank accession numbers for the sequences reported in this paper are AF327711, AF327712, AF327713, AF327714, AF327715, AF327716, AF327717, AF327718, AF327719 and AF327720.Microbiology, 2001
- Biosynthesis of the methanogenic cofactorsPublished by Elsevier ,2001
- Novel Formaldehyde-Activating Enzyme in Methylobacterium extorquens AM1 Required for Growth on MethanolJournal of Bacteriology, 2000
- Identification of triphosphoribosyl‐dephospho‐CoA as precursor of the citrate lyase prosthetic groupFEBS Letters, 2000
- Characterization of a second methylene tetrahydromethanopterin dehydrogenase from Methylobacterium extorquens AM1European Journal of Biochemistry, 2000
- Identifying two ancient enzymes in Archaea using predicted secondary structure alignment.Nature Structural & Molecular Biology, 1999