Leukemic Serum Inhibition of Phytohemagglutinin-Induced Transformation

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Abstract
The influence of leukemic serum on phytohemagglutinin-P (PHA-P)-induced lymphocyte transformation was studied. Twenty of 22 pretreatment sera collected at diagnosis inhibited the transformation of normal lymphocytes stimulated with PHA-P by more than 50%. In these experiments with PHA-P, kinetic studies of transformation demonstrated that leukemic serum inhibited rather than delayed transformation. The inhibition of transformation by PHA-P was assumed to be due to binding of PHA by components of leukemic serum because the inhibition could be overcome by an increase in the PHA concentration. A biologic assay of the PHA content of lysates of lymphocytes preincubated with PHA and leukemic serum further substantiated the PHA-binding mechanism of leukemic serum. Whether the differences observed in the binding capacity of normal and leukemic serum are qualitative or quantitative remains to be determined.

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