Demonstration, Mass Production and Characterization of a Heavy Molecular Weight Human Interferon

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Abstract
Summary: The present study demonstrates that it is possible to produce interferon in vitro using human amniotic membrane infected with various myxoviruses. Interferon of human origin thus can be obtained without cell culture. After viral inoculation, interferon is eliminated into the culture medium for approximately 5 days. In most instances a maximum yield is obtained 48 hr after viral inoculation. The yield of interferon is comparable to the yield of interferon produced by human leukocytes when Sendai virus is used as the inducing virus. Ultraviolet irradiation of the virus increases the yield of interferon. Optimal yields of interferon are obtained at pH 7.4 and at 37°C. The biophysical properties of this interferon are different from those described for leukocytes employing the same inducing virus. The molecular weight is estimated to be 160,000. Although its mode of action has not been clearly defined like that of other interferons. HAM interferon acts during the vegetative phase of viral multiplication and for its action requires cellular RNA and protein synthesis.

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