Thermotolerance and the Heat-shock Response in Candida albicans

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Abstract
At elevated temperatures, yeast cells of Candida albicans synthesized nine heat-shock proteins (HSPs) with apparent molecular masses of 98, 85, 81, 76, 72, 54, 34, 26 and 18 kDa. The optimum temperature for the heat-shock response was 45.degree.C although HSPs were detected throughout the range 41-46.degree.C. Protein synthesis was not observed in cells kept at 48.degree.C. Yeast cells survived exposure to an otherwise lethal temperature of 55.degree.C when they had previously been exposed to 45 .degree.C. The thermotolerance induced during incubation at 45 .degree.C required protein synthesis, since protection was markedly reduced by trichodermin. Mercury ions induced a set of three stress proteins, one of which corresponded in size to an HSP, and cadmium ions evoked one stress protein seemingly unrelated to the HSPs observed after temperature shift.

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