Modification of a laser‐based flow cytometer for high‐resolution DNA analysis of mammalian spermatozoa
Open Access
- 1 May 1986
- Vol. 7 (3) , 268-273
- https://doi.org/10.1002/cyto.990070307
Abstract
Modification of a Coulter EPICS V orthogonal laser‐based flow cytometer/cell sorter allows resolution of X and Y mammalian sperm populations based on DNA content. The modification consists of beveling the sample injection tube situated in the flow chamber, adding a second fluorescence detector directly forward along the laser beam axis, and routing the collected fluorescence through an optical fiber bundle to one of the existing photomultiplier tubes. The X and Y chromosome‐bearing spermatozoa from the bull, boar, and ram can be resolved using this system.Keywords
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