The subcellular loclization and properties of the ferrochelatase of etiolated barley

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Abstract
The etioplast fraction prepared from dark-grown barley (Hordeum vulgare) contained the enzyme ferrochelatase. A mitochondrial fraction prepared from the same dark-grown tissue also contained ferrochelatase. After density-gradient centrifugation an etioplast band was collected that was free from detectable mitochondrial marker enzymes and yet retained ferrochelatase activity. A membrane band that was enriched in mitochondria also contained ferrochelatase. The ferrochelatase in these 2 bands had different pH optima, but appeared very similar in their porphyrin specificity and their inhibition by metalloporphyrins.