Transcriptional regulation of immunoglobulin expression in a chronic lymphocytic leukemia cell line

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Abstract
A cell line derived from a B‐type chronic lymphocytic leukemia, Corinna II, was found to down‐regulate its steady‐state level of IgM mRNA after treatment with phorbol 12‐myristate 13‐acetate while the proliferative capacity of the cell line was unaffected. No changes in the splicing pattern of the IgM transcript could be observed after treatment. The down‐regulatory effect on IgM RNA expression was found to be inhibited by cycloheximide, suggesting that functional protein synthesis was needed for the effect. Transfection experiments showed that the down‐regulatory effect of phorbol myristate acetate was exerted at the level of transcriptional initiation. The DNA element mediating the down‐regulatory effect was found to be present within 140 bp 5' of the mRNA cap site in an immunoglobulin promoter.

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