The interaction of substituted 2‐phenylquinoline intercalates with poly(A) · poly(U): Classical and threading intercalation modes with RNA
- 1 January 1994
- journal article
- research article
- Published by Wiley in Biopolymers
- Vol. 34 (1) , 61-73
- https://doi.org/10.1002/bip.360340108
Abstract
The interaction of a series of 2‐phenylquinoline derivatives with RNA was investigated by means of viscometric, pKa, spectroscopic, binding, Tm, and kinetic methods. Compounds 1,2, and 3 have a piperazyl substituent at the para, meta, or ortho position, respectively, while 4 has an unsubstituted phenyl ring. The pKa results suggest that 1 has three charges, 2 and 3 have more than two charges, and 4 has two charges at pH 6.2. Spectroscopic and Tm results indicate that 1 binds more strongly to RNA than 2–4. Kinetic and modeling results indicate that 1 is a threading intercalator while 2 and 4 are classical intercalators. All experimental results indicate that 3, which has a large twist between the phenyl and quinoline rings, binds weakly with RNA. © 1994 John Wiley & Sons, Inc.Keywords
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