Immortal Time: Circadian Clock Properties of Rat Suprachiasmatic Cell Lines
- 29 January 1999
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 283 (5402) , 693-695
- https://doi.org/10.1126/science.283.5402.693
Abstract
Cell lines derived from the rat suprachiasmatic nucleus (SCN) were screened for circadian clock properties distinctive of the SCN in situ. Immortalized SCN cells generated robust rhythms in uptake of the metabolic marker 2-deoxyglucose and in their content of neurotrophins. The phase relationship between these rhythms in vitro was identical to that exhibited by the SCN in vivo. Transplantation of SCN cell lines, but not mesencephalic or fibroblast lines, restored the circadian activity rhythm in arrhythmic, SCN-lesioned rats. Thus, distinctive oscillator, pacemaker, and clock properties of the SCN are not only retained but also maintained in an appropriate circadian phase relationship by immortalized SCN progenitors.Keywords
This publication has 12 references indexed in Scilit:
- A Serum Shock Induces Circadian Gene Expression in Mammalian Tissue Culture CellsPublished by Elsevier ,1998
- Three period Homologs in Mammals: Differential Light Responses in the Suprachiasmatic Circadian Clock and Oscillating Transcripts Outside of BrainNeuron, 1998
- Expression of Brain-Derived Neurotrophic Factor and Its Cognate Receptor, TrkB, in the Rat Suprachiasmatic NucleusExperimental Neurology, 1998
- Circadian rhythm of brain-derived neurotrophic factor in the rat suprachiasmatic nucleusNeuroscience Letters, 1998
- Two period Homologs: Circadian Expression and Photic Regulation in the Suprachiasmatic NucleiNeuron, 1997
- Light-Induced Resetting of a Mammalian Circadian Clock Is Associated with Rapid Induction of the TranscriptCell, 1997
- A Differential Response of Two Putative Mammalian Circadian Regulators, mper1and mper2, to LightCell, 1997
- Circadian oscillation of a mammalian homologue of the Drosophila period geneNature, 1997
- Kinetic Model of 2-Deoxyglucose Metabolism Using Brain SlicesJournal of Cerebral Blood Flow & Metabolism, 1990
- Rhythm of suprachiasmatic nucleus 2-deoxyglucose uptake in vitroBrain Research, 1986